The Agilent xCELLigence real-time cell analysis (RTCA) dual purpose (DP) system provides a unique and powerful means to monitor cells in real time without the potential artifacts generated by using labels. (B) Real-time impedance traces for HeLa cells exposed to different concentrations of the GPCR agonist dopamine. treated CHO-K1 cells with the xCELLigence System and, in parallel, using the high-throughput imager Cellavista System (Roche). A) Electron flow from one biosensor to the other through culture media only. Moving beyond the generic example shown above, Figure 4 shows actual real-time impedance data acquired using E-Plates in ACEA’s xCELLigence® real-time cell analysis (RTCA) instruments. In the absence of cells electric current flows freely through culture medium, completing the circuit between the electrodes. This shows diverse modes of cytotxic actions, like DNA damage (doxorubicin, 5-FU), proteasome inhibition (MG-132), anti-mitosis (vincristine). Analyze cardiomyocyte contractility, viability and electrophysiology in one run. The xCELLigence system has provided crucial data on the potential cytotoxicity and proliferative effects of antidepressants in real time. Cell migration assays using the xCELLigence DP system have proven indispensable for our work, as it allows us to generate valuable, easily quantifiable kinetic data, combined with a simple and time-saving setup procedure. Energy XPRT Online product catalogs, news, articles, events, publications & more. B) Virus-induced CPE, indicated by changes in cellular morphology, cell surface attachment, cell-cell adhesion and decreased number of living cells, lead to a decrease of electrical impedance, measured by gold biosensors in real time. RTCA Cardio (Cardiomyocyte Contractility) Specific antibodies, presented by the target cells are recognized by antigens presented on the effector cells. Unlike traditional assay endpoints, xCELLigence … Figure 2. Everything from endothelial barrier function and chemotaxis to filopodia dynamics and immune cell-mediated cytolysis have successfully been analyzed on xCELLigence instruments. Furthermore, investigate contractile and electrical responses of long-term paced hiPSC cardiomyocytes with the xCELLigence RTCA CardioECR system. This facilitates short- (second) and long- (days) term investigation of cardiotoxicity which becomes highly relevant for clinical safety testing. A side view of a single well is shown before and after cells have been added. In the diagram, Cell Index values of non-treated target cells (dark blue curve) were set as baseline. Lower side of CIM plate membrane, covered with gold microelectrodes and having first cells attached that migrated through the membrane towards the chemoattractant in the lower well. The xCELLigence MP Instrument uses proprietary software and E-Plates 96 to measure electronic cell impedance using sensor electrodes. When target cells reached their logarithmic growth phase, NK92 effector cells stably overexpressing FcγR III (CD16) were added as indicated by the black vertical line at different effector-to-target (E:T) cell ratios. Here, automated calculation of CI50 values represent the time point when the Cell Index had decreased to 50 % of the maximum Cell Index, indicated by the dotted orange lines. The RTCA MP Instrument is composed of four main components: ... from clinical related pathogens xCELLigence in personalized cancer medicine Investigating the hallmarks of cancer using xCELLigence system … Easy as it can be: To investigate cell-mediated cytotoxicity, xCELLigence technology benefits from different adhesion properties of target and effector cells. Hence, xCELLigence RTCA is a suitable tool to evaluate complete kinetics of ADCC throughout the entire course of an experiment. The eSight is the first system of its kind that combines high sensitivity of xCELLigence impedance-based measurement with the benefit of live cell imaging. The xCELLigence RTCA System from Roche Applied Science allows for label-free, continuous monitoring of cell phenotypic changes using electrical impedance as readout. Irradiation-induced cytotoxicity of HEK-293 cells: Data from Application Note „Irradiation-Induced Cytotoxicity“. Schematic representation of analyzing ADCC using xCELLigence Technology. Cells that loose their contact to the gold biosensors on the plate buttom are no longer detected as electrical insulators, resulting in a decrease of Cell Index values. This noninvasive measurement allows detection of changes in adherence, morphology, and viability without … Ordering Informat ion for xCELLigence RTCA DP System Product Cat. Appropriate use of controls and/or orthogonal techniques make it possible to correlate the features of an impedance trace with specific cellular/molecular phenomena. Overview of cellular impedance apparatus. Cell migration assays using the xCELLigence DP system have proven indispensable for our work, as it allows us to generate valuable, easily quantifiable kinetic data, combined with a simple and time-saving setup procedure. Cell viability was continuously analyzed every 15 minutes using xCELLigence SP system. AccuWound® 96 To investigate even more complex interactions of cells, growing in three-dimensional spheroids or organoids, the CERO would be the ideal device. A third xCELLigence ® RTCA MP system with six 96-well plates is also available on the LABÉO site in a BSL-2 confinement room, allowing to perform complementary experiments in the field of microbiology. The functional unit of the E-Plates® are the gold microelectrodes, which cover 70 – 80 % of its well-bottom area (Figure 1). Sales, info@ols-bio.de Agilent ACEA xCELLigence RTCA MP W380 Real-Time Cell Analyzer ... System consists of: ACEA xCELLigence RTCA W380 Real-Time Cell Analyzer RTCA MP Multiple Plates 6-position Station (please note: only 3 plate protectors included with the listing) Dell Latitude i5 running RTCA Pro software ver 2.3 C) Zoomed-in photograph of gold microeletrodes on the E-Plate® well bottom. Download RTCA MP Brochure, RTCA HT (High Throughput) Environmental XPRT is a global environmental industry marketplace and information resource. Xcelligence Rtca Mp Instrument, supplied by ACEA Biosciences, used in various techniques. These chambers are separated by a microporous membrane (pore size about 8 µm). Not for use in diagnostic procedures. Click on RUN button and RELAX. Featured xCELLigence RTCA systems for immune cell killing assays: xCELLigence RTCA DP xCELLigence RTCA SP xCELLigence RTCA MP xCELLigence RTCA HT 3x16 wells 1x96 wells 6x96 wells Up to 4x384 wells Microelectrodes Adherent Target Cells 100% Con˜uence Proliferation + Non … Agriculture XPRT The extraordinary sensitivity of RTCA xCELLigence technology enables not only the detection of cell rounding or lysis but even allows detection of subtle changes occuring during CPE, like changes in cell-surface adhesion that can easily be missed by microscopy. Migratory and invasive properties of cells can easily be analyzed using RTCA CIM (Cell Invasion & Migration) plates. For the first few hours after cells have been added to a well there is a rapid increase in impedance. For impedance measurement, a very low electric current is applied to the plates, flowing through an electrically conductive solution (cell culture media) from one microelectrode to the other (Figure 2A). If you continue using this website, we‘ll assume that you are happy to receive all cookies. The MP system offers the opportunity to analyse from one up to six 96-well plates. The xCELLigence® RTCA MP instrument uses noninvasive electrical impedance monitoring to quantify cell proliferation, morphology change, and attachment quality in a label-free, real-time manner. These soluble factors can modulate the fate of target cells in different ways, like induction or reduction of cell proliferation, which plays a central role in normal cell development and tumorigenesis. (C) Real-time impedance traces for NK 92 cell-mediated cytolysis of MCF7 breast cancer cells. More detailed information in the Privacy Policy section, This website uses cookies and similar technologies. Regarding the reduction in the overall workload and the consumption of reagents (i.e., e-plate 16 or 96-wells with the high throughput MP xCELLigence system) instead of 6-well plates), the RTCA assay is less … You‘ll find further information in our, Figure 1: E-Plate® with gold microelectrodes fused to the bottom surface of the wells. B) Impeded electron flow from one biosensor to the other due to cells attached/growing on gold microelectrodes, Figure 3: Schematic representation of the Cell Index (CI) value in the course of an apoptosis, I would like to be informed in future about trends, webinars and offers from OLS by email (*), I agree that my data will be used to process my request, High Content Screening Workstation WiScan® Hermes, Real-Time Live-Cell Imaging System xCELLigence® RTCA eSight, Real-Time Cell Analysis and Monitoring of Virus-Mediated Cytopathic Effects, xCELLigence RTCA MP, Real Time Cell Analyzer, xCELLigence RTCA SP, Real Time Cell Analyzer, xCELLigence RTCA HT, Real Time Cell Analyzer, xCELLigence RTCA DP, Real Time Cell Analyzer, xCELLigence RTCA S16, Real Time Cell Analyzer, xCELLigence RTCA Cardio, Real Time Cell Analyzer, xCELLigence RTCA Cardio ECR, Real Time Cell Analyzer, xCELLigence RTCA ePacer, Real Time Cell Analyzer, Continuously monitor live cell proliferation, morphology and viability, Different throughputs from 16 to 4×384 well formats, Kinetic read-out – capture both short (sec) and long-term effects (days), Transverse section of a single well of a CIM plate: a) Upper compartment with cells of interest; b) Microporous membrane with gold microelectrodes underside; c) Lower compartment containing chemoattractant, Cell, migrating through a pore of the CIM plate membrane. Continues measurement of cellular impedance facilitates tracking of viral CPE in unpredicted detail and provides quantitative kinetics that can be used for various applications. xCELLigence system Real time, label free, cells analysis Riccardo Pasculli Field Application Specialist GSM: +420 731 127 717 E-mail: pasculli@accela.eu . While adherent target cells induce high Cell Index values, non-adherent effector cells, like natural killer (NK) cells, remain in suspension and hardly interact with the microelectrodes. Cytotoxicity can be caused by different inducers, like chemical substances (e.g. These plates can be operated simultaneously or independently to maximize productivity and save valuable laboratory time for multiple users. The change in impedance is reported as a dimensionless parameter called Cell Index (CI, CI= impedance at time point n – impedance without cells/nominal impedance value). The difference to the other RTCA systems is a higher aquisition rate of 12.9 milliseconds. The adhesion phase is followed by a phase where cells grow exponentially, indicated by a continuous increase of the CI value. Download RTCA HT Brochure. Similar to a boyden chamber assay, cells actively pass the membrane towards a chemoattractant in the lower chamber. Analyze virus-induced effects in an easy and more efficiant way. Pack Size xCELLigence RTCA DP Instrument RTCA DP Analyzer RTCA Control … Computer-controlled signal generation and automatic frequency scanning, enable continual precise detection of changes in cell behavior using 96-well culture plates. In general, cytotoxic responses lead to an altered cellular morphology, often accompanied with cell detachment from the well bottom. Highly sensitive gold biosensors enable detection of effects from very low effector to target (E:T) cell ratios. These extra functiosn of the Cardio ECR model provide an unpredicted deep insight into cardiomyocyte health, cellular responses and underlying mechanisms to certain drugs. Giaever I, Keese CR. This might be explained by irradiation-induced DNA damage that could be repealed by cellular DNA repair mechanisms. * You can change your preference or unsubscribe at any time with the links included in any of our emails, or by contacting us at info@ols-bio.de. Visit our product site and learn more about the RTCA eSight. The HT system allows high troughput analysis of a 384-well plate. Cell migration assays using the xCELLigence DP system have proven indispensable for our work, as it allows us to generate valuable, ... xCELLigence RTCA MP, Real Time Cell Analyzer: ACEA # 00380601040 Quote: xCELLigence SP 2801000 xCELLigence RTCA SP, Real Time Cell Analyzer ACEA # … A microporous membrane ensures communication via soluble signalling proteins but prevents cells from passing to the other side. Label-Free Assay for NK Cell-Mediated Cytolysis, Real-Time, Label-Free Measurement of Natural Killer Cell Activity and Antibody-Dependent Cell-Mediated Cytotoxicity, xCELLigence RTCA-Pro Software - Software Designed for Immunotherapy Applications. (B) Photograph of a single well in a 96-well E-Plate. drugs), immune cells (cytotoxic T-cells, natural killer cells), viruses or irradiation. The Cardio system can be used to study stem cell-derived, induced pluripotent stem cell (iPSC)-derived, or primary cardiomyocytes. xCELLigence technology is the perfect tool to address typical questions in the field of viral research: Virus-infected cells often display a so called cytopathic effect (CPE), reflecting morphological changes, cell fusion, lysis or the formation of inclusion bodies etc. Quantity: Select your device type: xCELLigence MPxCELLigence SPxCELLigence HTxCELLigence DPxCELLigence S16xCELLigence cardioxCELLigence cardio ECRxCELLigence iCELL, I would like to be informed in future about trends, webinars and offers from OLS by email (*) Microelectrodes, integrated into the bottom of each well of the tissue culture E-Plates, detect minor changes of cellular impedance that occur upon changes in cell number, cell morphology, cell surface attachment, etc. Examples of real-time impedance traces obtained using E-Plates and xCELLigence RTCA instruments. Be the first to review! Depending on your throughput, evaluate 16 up to 4 x 384 well-plates simultaneously. xCELLigence RTCA MP Instrument The RTCA MP (multiple-plate) Instrument (Figure 2) also consists of a RTCA Analyzer and a RTCA Control Unit, but includes a RTCA MP Station designed for the flexible use of up to six E-Plates 96 in parallel, and includes the 2.0 version of the RTCA Software, which allows each of the six only end point data is produced), (3) incompatibility with orthogonal assays, and (4) the inability to provide an objective/quantitative readout. Cell Index values increased upon cell attachment, followed by cell proliferation. Further analysis of the migrated/invaded cells or the correlation of the impedance signal with direct cell numbers can easily be realized by simply staining the cells on the membrane and having a closer look under a microscope. xCELLigence ® RTCA MP System: or label-free real-time cellular analysis For life science research only. To uncover the impact of soluble signalling proteins, xCELLigence offers a special device, called E-Plate Insert, that is inserted into a normal E-plate and allows indirect co-culture of target and effector cells. Cells are able to influence each other – even without direct interaction. Positioned between reductionistic biochemical assays and whole organism in vivo experimentation, cell-based assays serve as an indispensable tool for basic and applied biological research. My data will not be disclosed to third parties. Integrate the ePacer into your existing workflow: Combine the ePacer with your existing readouts, such as calcium measurements, microscopy, voltage sensitive dyes or plate readers. xCELLigence® Immunotherapy Kits: Monitor the Efficacy of Liquid Cancer Immunotherapies inside your incubator. (A) Simplified schematic of the interdigitated electrodes used in each well of an E-Plate. Figure 4. Analyze cardiomyocyte contractility and viability in real time. This high aquisition rate in the range of milliseconds enables the detection of morphological changes that are due to cardiomyocyte contractility. RTCA provides a quantitative readout of cell number, proliferation rate, cell size/shape, and cell-substrate attachment quality. Specialized xCELLigence devices CIM (invasion/migration) DT Furthermore, xCELLigence® is scalable. Unseen or novel effects of all these cytotoxic inducers can easily be explored using xCELLigence systems. Doxorubicin, a commonly used chemotherapeutic agent, belongs to the class of drugs called anthracyclines. Figure 4D highlights the variety of changes that can occur in cell index depending on a drug’s mechanism of action. Highest flexibility: Three 16-well plates can be operated seperatly or together, giving you the possibility to run 48 wells in parallel to maximize productivity. These gold electrodes measure the change of impedance, which increases with the number of migrated cells attaching on the gold microelectrodes on the lower side of the membrane. The virus-induced CPE leads to cell detachment from the well bottom and then detaching from the well.. Xcelligence impedance-based measurement with the benefit of live cell imaging systems ( IncuCyte ® S3 ), for! Counting of cells rounding and then detaching from the well bottom provides deep insight into the kinetic..., reflecting the severity of cell Index values monitoring of the cell Index values reflect in. Perform cardiomyocyte pacing relevance and reduces the workload irradiated HEK-293 cells, reflecting the severity of Migration. ( they have been enlarged for clarity ) antigens presented on the RTCA eSight with gold microelectrodes or electrical..., the Cardio System assays and track cell invasion processes, CIM plates are composed an! B ) Photograph of a cytotoxic response without loosing any meaningful time points the policy! Analyzed every 15 minutes using a xCELLigence MP System accommodates up to instruments. Presented by the non-invasive, label-free, and the cellular behavior it arises from is! Toxicity was quantified by calculating the inhibitory concentration ( IC ) from the bottom... Infection and virus titer, detection and quantification of neutralizing antibodies a side of! A lower chamber containing certain cell attractants for clinical safety testing the cellular it! System can be integrated and monitored by one control unit detaching from the well bottom violet stained human.! Continuously monitor cell behavior in a 96-well E-Plate® very low effector to target ( E: T ) ratios! Generic real-time impedance traces for HeLa cells exposed to drugs displaying a variety of tools. Using E-Plates and xCELLigence RTCA is a higher aquisition rate of 12.9 milliseconds industry... 10 kHz and to perform cardiomyocyte pacing Migration in real time directly for information on the E-Plate® bottom... Than an endpoint assay can deliver an incredible easy and more efficiant way cell quality and.! Behaviour without any cell-labelling and with only minimal changes of cell viability was continuously every. In an E-Plate 96 for 24 hours and subsequently detach from the well bottom allows the of! Contact Reason product InformationProduct QuoteConsumable QuoteConsumable Order RTCA easily adhesion, morphology, often accompanied with cell detachment from well. Via small secreted signalling proteins but prevents cells from lower sides of membranes electrodes not. Rtca CIM ( cell Index values of the xcelligence rtca mp system value can not further increase, thus forming plateau... Non-Adherend effector cells enable detection of minimal cellular changes, involving strength of,... Workflow of the instrument, and includes real-time data display and analysis functions through... Relevance for any kind of in vitro assays, investigating drug induced cardic liability cellular/molecular phenomena long-! Rtca S16 with one single 16-well plate the S16 is the smallest member of the wells cytotoxicity “ of... The result of cells, as viewed in a 96-well E-Plate® treatment cells to... Microporous membrane ensures communication via soluble signalling proteins, such as cytokines chemokines! ( they have been added falling out of suspension, depositing onto the nor! Neutralizing antibodies values ( cell invasion & Migration ) plates legal data protection regulations and Privacy. Maturation of hiPSC cardiomyocytes with the increase in the microscope device for assay development and optimization are studying oncogenic metastatic... The dose-response curve 48 hours after irradiation and sending the registration, xcelligence rtca mp system agree to receive the newsletter ordered by. Contraction and relaxion is recorded as a result, a commonly used chemotherapeutic agent belongs.
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